Freethought & Rationalism Archive

Amit Misra · 06-06-2003, 02:34 AM

Missed this thread during its April stint.
I guess RNAi is neat as an experimental tool, but Lost Girl's take on it is quite perceptive.
Even more than dsDNA or ssRNA, dsRNA will be the very devil to deliver to the target cell's ER. DNA delivery using viral and non-viral vectors has problems enough.

Wounded King · 06-06-2003, 03:04 AM

Why should dsRNA be more tricky than ssRNA? I thought that the double stranded form was more stable. The best thing would probably be not to use the dsRNA itself anyway but a DNA coding for an mRNA with a hairpin or coding seperate sense and antisense strands for the target sequence.

Here is an interesting review

Reuven Agami
RNAi and related mechanisms and their potential use for therapy
Current Opinion in Chemical Biology
Volume 6, Issue 6 , 1 December 2002, Pages 829-834

Wounded King · 06-19-2003, 04:54 AM

Forget medical science what about consumerism.

New Scientist has an article about low-caffeine producing coffee plants where the caffeine producing genes are silenced by transgenic RNAi.

http://www.newscientist.com/news/news.jsp?id=ns99993851

06-06-2003, 02:34 AM	#21
Amit Misra Senior Member Join Date: Jun 2000 Location: Lucknow, UP, India Posts: 814	The Pharmacist's Challenge! Missed this thread during its April stint. I guess RNAi is neat as an experimental tool, but Lost Girl's take on it is quite perceptive. Even more than dsDNA or ssRNA, dsRNA will be the very devil to deliver to the target cell's ER. DNA delivery using viral and non-viral vectors has problems enough.

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06-06-2003, 03:04 AM	#22
Wounded King Veteran Member Join Date: Mar 2003 Location: Edinburgh Posts: 1,211	Why should dsRNA be more tricky than ssRNA? I thought that the double stranded form was more stable. The best thing would probably be not to use the dsRNA itself anyway but a DNA coding for an mRNA with a hairpin or coding seperate sense and antisense strands for the target sequence. Here is an interesting review Reuven Agami RNAi and related mechanisms and their potential use for therapy Current Opinion in Chemical Biology Volume 6, Issue 6 , 1 December 2002, Pages 829-834

06-19-2003, 04:54 AM	#23
Wounded King Veteran Member Join Date: Mar 2003 Location: Edinburgh Posts: 1,211	Forget medical science what about consumerism. New Scientist has an article about low-caffeine producing coffee plants where the caffeine producing genes are silenced by transgenic RNAi. http://www.newscientist.com/news/news.jsp?id=ns99993851

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